Regulatory

Part:BBa_K2285020:Design

Designed by: Zhuoyang Chen   Group: iGEM17_SJTU-BioX-Shanghai   (2017-10-24)


J23119+Target3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any CDS such as reporter like sfGFP and chromoprotein or other functional gene can be added to achieve greater and more convenient control. Make sure that Antisense of Star3 system are also included in the same bacteria to activate downstream gene expression of Target3.


Source

The Star3 system including Target1 and Antisense1 is originated from the T181 strain (Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs.) with certain modifications and improvements.

References